Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination - Supplementary Material

Aim: Ova and parasite examination by flotation requires hypertonic solutions, which can damage the
egg and cyst membranes, leading to false negatives. The authors investigated the harmful effects of
ZnSO4 and C12H22O11 solutions on the ova and parasite examination. Materials & methods: The authors
processed samples using the Three Fecal Test technique. Aliquots were floated in different pH levels,
temperatures and solution densities. Results: Densities above 1.12 g/ml led structures to collapse after 6–
10 min. pH neutralization of the ZnSO4 solution did not prevent the parasites from changing. Conclusion:
All structures were altered when standard methods were performed. To delay collapse, the parasite
floating under 5 ◦C is highly desirable.