Homo sapiens strain:Peripheral Blood | isolate:SA-Beta-beta-Galactosidase-CD34-Positive Raw sequence reads

Senescent and Active-HSPCs were isolated from three healthy individuals (ages 34, 50 and 52 years). Total RNA was purified from FACS-isolated cells from each population using the Zymo Research Quick-RNA MicroPrep following manufacturer's protocol. Quality and purity of the RNA was determined in Agilent Bioanalyzer analysis using the RNA 6000 Pico kit. A total of 5 ng of RNA was used for library generation. Library generation was performed using the Nugen Ovation RNA-Seq System V2. Sequencing was done using an Illumina HiSeq 2500 with 75 base pair, paired-end reads. Six samples were multiplexed into one HiSeq 2500 lane.