Summary of all the differentiation experiments with the 5 differentiation media (DifM1-5)*.

*A single isolated spheroid was induced to attach to the gelatine-coated (DifM1,2,3 and 5) or poylysine-coated (DifM4) well with serum for one day, and induced to differentiate during 15 days in the presence of any of the DifM 1 to 5. Wells were fixed and immunofluorescence performed. In some wells double immunofluorescence was performed (aligned in the same row): those wells where more than one cell type co-existed are written in bold and the number of cells double positives for both markers is shown in brackets; those wells where one single type of cell was detected are in italics. In some of the wells (underlined), co-immunofluorescence for GFRa2 (a) or Prop (b) was performed, always with a negative result.