K36me3 levels in WT and sdg7 sdg8. Arabidopsis thaliana

Polycomb Repressive Complex 2 (PRC2) recognizes Polycomb response elements (PREs) and catalyzes trimethylation of histone H3 on lysine 27 (H3K27me3) for gene silencing. This silencing is counteracted by H3K36 methylation for epigenetic activation of gene expression. The Arabidopsis thaliana H3K36 methyltransferases SET DOMAIN-CONTAINING PROTEIN 7 (SDG7) and SDG8 antagonize PRC2-mediated silencing and establish H3K36 methylation patterns with the general transcription machinery. Here, we performed H3K36me3 ChIP seq in WT and sdg7 sdg8 mutants. The sdg7 sdg8 double mutant shows developmental defects and lower H3K36me2 and H3K36me3 levels.