Profiling of N6-methyladenosine in mouse embryonic stem cells: Setd2 knockdown and control cells

Setd2 is the specific methyltransferase of H3K36me3. To understand the global effect of H3K36me3 on m6A modification, we used mouse embryonic stem cells (mESCs) model with doxycycline (Dox)-induced Setd2 knockdown, and performed m6A-IP followed by sequencing in mESCs with or without Dox treatment. We found that depletion of H3K36me3 by Setd2 silencing globally reduced m6A in mouse transcriptome. m6A-seq in mESCs with or without Dox-induced Setd2 knockdown.