A High-Resolution Map Predicts Lineage and Birth Order Underlying Neuronal Identity

We have used single-cell RNA-sequencing to profile dissected central brains from female and male adult Drosophila melanogaster. Overall design: Virgin female and male flies were collected and stored individually until the age of 5 days post-eclosion at 25°C prior to dissection. Two female replicates and two male replicates were processed with 20 brains each. The flies were transgenically expressing GFP under the control of the gene doublesex.