Spearman and partial Spearman correlations between ViennaRNA stability predictions at different gene regions and transgene protein/RNA levels.

Transgene data from Cambray and colleagues [41]. “stability_first10” refers to predicted stability in the first 30 bp in each transgene construct after the start codon (codons 2–11), “stability_mid” to predicted stability of the following 30 bp section within each transgene construct (codons 12–21), “stability_last10” to the 30 bp following that (codons 22–31). Partial Spearman correlations are controlling for the influence of stability in the other construct regions such that, for instance, the relation between transgene expression (as protein/RNA) and predicted RNA stability in region stability_first10 is found independently of the relation with predicted RNA stability in regions stability_mid and stability_last10. Number of start indicates significance such that p p p p p > 0.05 = no stars. The data underlying this Figure can be found in https://doi.org/10.5281/zenodo.17378284. (CSV)