Single cell RNA sequencing from extra-thymic Aire-expressing cells (Cd31-gp38-Cd45+EpCam+MHC+GFP+) sorted from mixed/pooled lymph nodes from 2 C57 BL/6 mice (Homunculus project)

Aire-expressing cells have been detected across several non-thymic immune sites, including peripheral lymph nodes. The transcriptome of these extra-thymic Aire expressing cells (eTACs) will be used to indicate their ontogeny and predicted function and antigen presenting cells. In particular, it is not known whether these cells represent a single homogenous population, or a comprised of multiple different cell types. Single cell RNA sequencing allows us to characterise any heterogeneity in these cells.