Effects of experimental warming on oak defenses and herbivory across latitudes

Forest microclimatic variation can result in substantial temperature differences at local scales with concomitant impacts on plant defences and herbivory. Such microclimatic effects, however, may differ across abiotically contrasting sites depending on background environmental differences. To test these cross-scale effects shaping species' ecological and evolutionary responses, we experimentally tested the effects of aboveground microhabitat warming on insect leaf herbivory and leaf defences (toughness, phenolic compounds) for saplings of sessile oak (Quercus petraea) across two abiotically contrasting sites spanning 9° latitude. We found higher levels of herbivory at the low-latitude site, but leaf traits showed mixed patterns across sites. Toughness and condensed tannins were higher at the high-latitude site, whereas hydrolysable tannins and hydroxycinnamic acids were higher at the low-latitude site. At the microhabitat scale, experimental warming increased herbivory but did not affec..., , , # Oak warming treatment

[https://doi.org/10.5061/dryad.8931zcrx4](https://doi.org/10.5061/dryad.8931zcrx4)

We conducted a manipulative field study testing for the effects of experimental warming at the microhabitat-scale on insect leaf herbivory and leaf traits associated with herbivory across two sites with contrasting (e.g., macroclimatic) conditions using sessile oak (*Quercus petraea*) as model species, a common tree species in many Western European broadleaf forests (Supporting Information). Specifically, we established two field sites spanning two thirds of this species’ latitudinal range, one in Spain (42°N) and one in Belgium (51°N), using three-year-old saplings local to each site. We imposed microhabitat-level warming for half of the saplings at each site using infrared heater arrays. After a full growing season (March to September 2022), we collected leaves to measure insect leaf herbivory and to quantify physical (specific leaf area, correlated with leaf thickness or tough...