RNA-Seq of Anopheles coluzzii infected with Plasmodium berghei, at various time points.. Transcriptional responses of Anopheles coluzzii to Plasmodium berghei infections

The availability of the genome sequence of the African malaria vector An. coluzzii (Anopheles gambiae M molecular form) has opened new opportunities to dissect at the molecular level the vector-pathogen interactions leading to malaria transmission. Here, we describe the characterization of the genome-wide transcriptional responses of An. coluzzii to infection with Plasmodium berghei laboratory isolates. Colonized An. coluzzii mosquitoes were fed on anesthetized mice infected with P. berghei, and the abundance of transcripts in the mosquito midgut was monitored at several time points post infection.