Figure 1. UL12.5 does not affect lytic replication in peripheral neurons.

Titers of virus [PFU/mL]1A.Viral titers from primary sympathetic neurons (SCGs) quantified at 24 hours post-infection (at an MOI of 10 PFU/cell) KOS-SPA or KOS-UL98.1C.Viral titers from dermal fibroblasts) quantified at 24 hours post-infection (at an MOI of 3 PFU/cell) with KOS-SPA or KOS-UL98.qPCR data for;1B. Viral genome (vDNA) copy number, KOS-SPA (WT) or KOS-UL98 (UL12.5 null), 24 hours after infection, primary sympathetic neurons (SCGs).1D. Relative abundance of mtDNA (mtDLoop1) transcripts measured by (RT-) qPCR 12 hours after infection with KOS-SPA or KOS-UL98, primary sympathetic neurons (SCGs).1E. Relative abundance of mtRNA (mtCOX2) transcripts measured by (RT-) qPCR 12 hours after infection with KOS-SPA or KOS-UL98, primary sympathetic neurons (SCGs).1F. Relative Ifnb mRNA expression 24 hours after infection with KOS-SPA or KOS-UL98 of primarysympathetic neurons (SCGs).1G. Relative Ifnb mRNA expression 24 hours after infection with KOS-SPA or KOS-UL98 of primarydermal fibroblasts.1H. Representative immunofluorescence images (TIFs) of STING/TGN46 at 3 days post-transduction with either GFP or UL12.5 expressing lentiviral vector. Dataset showing STING and TGN46 (Golgi) area (px2) and calculated percentage of STING area overlap with Golgi for each condition. Measurements performed in Fiji.