Caco-2 cell permeability analysis using transepithelial electrical resistance (TEER).
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aCaco-2 cells monolayers were grown in transwell inserts and treated with wild type (WT) or Listeria adhesion protein (LAP)-expressing Lb. paracasei for 1, 4, 15, and 24 h, then treated with L. monocytogenes for 2 h. TEER measurements before and after L. monocytogenes treatment alone were 279.40±1.19 and 243.57±1.20, respectively. Values are averages of two experiments analyzed in triplicate and are significantly different between LbpWT and LbpLAP at all time points (Pafter ÷ TEERbefore ×100.
创建时间:
2015-12-02



