Transcriptomic analysis of Fusobacterium nucleatum-stimulated Human gingival fibroblasts

To better understand the global responses of GFs to F. nucleatum infection along the time sequence, we performed a genome-wide transcriptome analysis by RNA-sequencing technology to determine the global changes on gene expression level Overall design: 54 samples from 5 individuals (each individual involved 1 sample at time point 0h, 5 control samples at 2h 6h, 12h, 24h and 48h with no F. nucleatum treatment, and 5 experimental samples with F. nucleatum (F. nucleatum:cell=100:1) treatment at the same time point, one treated sample at time point 12h failed to extract sufficient RNA) were sequenced to analysis the whole gene expression level