Chromosome level assemblies and gapless centromeres of Arabidopsis thaliana accessions Ey15-2 (9994) and Col-0 (6909) with PacBio long read sequencing technologies

To compare the performance of the current long-read sequencing platforms offered by PacBio, we generated CLR and HiFi libraries starting from the same high-molecular-weight DNA extraction of a pool of individuals of the Arabidopsis thaliana natural accession Ey15-2 (accession ID 9994; CS76399). We produced an optical map with the Bionano Direct Label and Stain (DLS) technology to validate and scaffold the main assemblies, and an Illumina PCR-free paired read library to evaluate completeness and accuracy of all assemblies, and to estimate the genome size of this particular strain. In addition, to compare gapless centromeres between different Arabidopsis thaliana accessions, we generated a HiFi library from a single individual of the accession Col-0 (accession ID 6909; CS76778).