Growth inhibition of glioma cells by hUCBSC.

(A) MTT assay of single and co-cultures of glioma cells with hUCBSC. Even though proliferation values of both single cultures of glioma cells and hUCBSC were taken as 100%, values of proliferation of glioma + hUCBSC cocultures were calculated in relation to single cultures of glioma alone. When we calculated values of proliferation of glioma + hUCBSC cocultures in relation to single cultures of hUCBSC, we got much less values compared to the values presented here. (B) Cytotoxicity assay based on cytosolic LDH assay. (C) FACS analysis results of glioma cells showing apoptotic cells as measured by Annexin V. Immunoblot analysis of proteins showing expression of XIAP, Akt and pAktSer473 in (D) single and co-cultures of glioma cells with hUCBSC and (E) single and co-cultures of glioma cells with astrocytes. 40 µg of proteins were loaded onto 12% gels and transferred onto nitrocellulose membranes and probed with respective antibodies. Immunoreactive bands were visualized using chemiluminescence ECL Western blotting detection reagents and the reaction was detected using Hyperfilm-MP autoradiography film. Each experiment was repeated 3 times. Error bars indicate ±SEM. * Significant at p<0.05.