miR-613 binds to the 3'UTR of the NR1H3 mRNA

Translation of liver X receptor α (LXRα or NR1H3) mRNA is negatively regulated by microRNA 613 (miR-613), which binds directly to the specific miRNA response element (613MRE) within the 3'UTR of NR1H3 mRNA (Ou Z et al. 2011; Zhong D et al. 2013). The expression of miR-613 itself was induced upon the activation of NR1H3. However, miR-613 did not appear to be a direct NR1H3 target gene (Ou Z et al. 2011). Instead, the positive regulation of miR-613 by NR1H3 was mediated by the sterol regulatory element binding protein (SREBP)-1c, a known NR1H2,3 target gene (Ou Z et al. 2011). The binding of miR-613 to NR1H3 mRNA repressed lipogenesis in human liver carcinoma HepG2 cells and this was reversed by NR1H3 overexpression (Zhong D et al. 2013).

肝X受体α（LXRα或NR1H3）mRNA的翻译受微RNA 613（miR-613）的负性调控，后者直接结合至NR1H3 mRNA的3'非编码区（3'UTR）内的特异性miRNA反应元件（613MRE）（Ou Z 等人，2011；Zhong D 等人，2013）。miR-613的表达在NR1H3激活后得到诱导。然而，miR-613似乎并非NR1H3的直接靶基因（Ou Z 等人，2011）。相反，NR1H3通过固醇调节元件结合蛋白（SREBP)-1c的正性调控miR-613，SREBP)-1c是已知的NR1H2,3靶基因（Ou Z 等人，2011）。miR-613与NR1H3 mRNA的结合抑制了人肝癌细胞HepG2中的脂质生成，而NR1H3过表达则逆转了这一抑制作用（Zhong D 等人，2013）。