RNA-seq profiling in metachronous colorectal liver metastasis

Differential expression analysis was performed on 36 metachronous colorectal liver metastases (mCLM) and adjacent non-malignant liver tissue sample pairs to identify deregulated genes. GSEA and CMS classification were used to explore pathways and stratify tumors. miRNA-mRNA interactions were investigated through co-expression and correlation analysis, with prognostic relevance assessed by survival analysis. This study gave a holistic view of the molecular landscape of mCLM and revealed key deregulated pathways and novel miRNA-mRNA interactions with potential prognostic implications.