Induced MIST1 and PTF1a Expression in Pancreatic Ductal Adenocarcinoma Cells

Purpose: The goal of the study was to determine the effects of forced expression of the acinar transcription factors MIST1 and PTF1a in PDAC cells. Methods: Doxycycline inducible MIST1myc and PTF1amyc Panc-1 cells were generated using Clontech's Tetone System and subjected to RNA-Sequencing following doxycycline treatment. Results: 50 million sequence reads were mapped to the human genome. Data suggests acinar associated molecules were induced upon doxycyline treatment. Conclusions: MIST1 and PTF1a retain functional activity and can promote acinar gene expression in the context of pancreatic cancer cells. Panc-1 tet-inducible MIST1 and PTF1a cells were treated in the absence or presence of 1 μg/ml doxycycyline for 72 hours.