Genome wide expression change by SMURF1 knocking down in MCF-7 cells

We aim to the investigate the role of SMURF1 in breast cancer progression. MCF-7 cells were used as the model and SMURF1 was silenced by siRNA. The MCF-7 cells were treated with 50nM stramble siRNA and siSMURF1. After 48 hours, cells were harvested and the total RNA was extacted by Qiagen kit. The RNA sample was sent to BGI for RNA expression anaylsis.