Multi-omics Investigation of Freeze Tolerance in the Amur Sleeper, an Aquatic Ectothermic Vertebrate（水生变温脊椎动物阿穆尔睡眠者的冷冻耐受性的多组学研究）

我们对葛氏鲈塘鳢的染色体水平基因组进行了测序，进行比较基因组学、转录组学和代谢组学分析，以研究其在冷冻中生存的策略。其最接近的非耐寒亲戚的差异约为 15.07 百万年前，经历了高速的蛋白质进化。转录组学和代谢组学数据识别加强了参与代谢低下、细胞代谢的基因和代谢物的协调和组织特异性调节应激反应以及冷冻和解冻过程中冷冻保护剂的积累。几个基因显示证据加速蛋白质序列进化或家族规模扩张被发现是对冻结的适应性反应诱发应力。具体来说，与细胞骨架稳定性、冷冻保护剂合成、反式膜运输和神经保护适应被认为是有 助于的潜在关键创新冻结生存。 我们的工作为揭示分子适应提供了宝贵的资源和机会移植变温脊椎动物的冷冻耐受性。

We sequenced the chromosome-level genome of *Perccottus glenni*, and performed comparative genomics, transcriptomic and metabolomic analyses to investigate its freeze survival strategies. This species diverged from its closest non-freeze-tolerant relative approximately 15.07 million years ago, with both lineages undergoing rapid protein sequence evolution. Transcriptomic and metabolomic analyses identified and validated genes and metabolites that mediate coordinated, tissue-specific regulatory programs for hypometabolism, cellular metabolic stress responses, and the accumulation of cryoprotectants throughout freezing and thawing cycles. Several genes showing evidence of accelerated protein sequence evolution or family expansion were found to be involved in adaptive responses to freezing-induced stress. Specifically, adaptations related to cytoskeletal stability, cryoprotectant synthesis, transmembrane transport, and neuroprotection are considered critical potential innovations supporting freeze survival. Our work offers a valuable resource and opportunity to uncover the molecular adaptations underlying freeze tolerance in poikilothermic vertebrates.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于水生变温脊椎动物阿穆尔睡眠者的冷冻耐受性，通过染色体水平基因组测序、比较基因组学、转录组学和代谢组学分析，揭示了其高速蛋白质进化以及基因和代谢物在冷冻过程中的协调调节。研究发现细胞骨架稳定性、冷冻保护剂合成等适应性反应是冷冻生存的关键创新，为理解变温脊椎动物的分子适应提供了宝贵资源。