Impact of RNA isolation methods for RNA-seq on gene expression. Here RNA was isolated from mouse striatal tissue using either Columns or magnetic beads and the isolated RNA was processed using prime-seq.. Impact of RNA isolation methods for RNA-seq on gene expression. Here RNA was isolated from mouse striatal tissue using either Columns or magnetic beads and the isolated RNA was processed using prime-seq.

Here silica column and bead based RNA isolation was performed. The two methods were compared using prime-seq to determine the effect of the RNA isolation method on gene expression quantification. This was tested for three different types of input samples, PBMCs, HEK293T cells and striatal mouse tissue to compare the RNA isolation over a range of inputs. For each sample and experimental condition 8 technical replicates were performed.