The Influence of One-Time Physical Activity at a Temperature of−10°C on Erythrocyte Deformability in Young Men - research data

The study group consisted of 30 healthy men, physiotherapy students at the University of Physical Culture in Krakow (Poland). Participants were randomly assigned to the experimental or control group using simple randomization with a 1:1 allocation ratio. The randomization sequence was generated prior to the study, and group assignment was performed before the first blood sampling. No stratification factors were applied.
The experimental group underwent physical exertion under low-temperature conditions. The exercise was held in a thermal climate chamber in the Laboratory for Climate Technology Research and Heavy Duty Machines, Cracow University of Technology (Krakow, Poland), at a temperature of −10 °C. The thermal climate chamber possesses an accreditation certificate No. AB 1678 was issued by the Polish Centre for Accreditation and meets the requirements of the PN-EN ISO/IEC 17025:2005 standard. The Laboratory for Climate Technology Research and Heavy Duty Machines is a research center accredited in the field of defense and security by the Minister of National Defence (accreditation No.: 55/MON/2018). The control group performed the same workout but in thermoneutral conditions, in the open air; on the day of the experiment, the air temperature was 18.1 °C.
The study comprised 3 stages. Firstly, blood samples were collected from all participants before the exercise. The blood collection was performed in the morning hours at the Blood Physiology Laboratory of the Central Research and Development Laboratory, University of Physical Culture in Krakow, on 11 May 2024. Subsequently, both groups proceeded to perform an exertion test. After the exercise, blood samples were taken from all study participants.