Kinetic characterisation of GCK-MODY mutations.

Data are given as mean ±SEM measured in n≥12 experiments. GCK-GST enzymes were prepared as 3 (WT)/2 (G258R)/1 (others) independent protein expressions. Glucose S0.5 values were normally measured in the glucose range 0–100 mmol/L with 5 mmol/L ATP, however for some mutant enzymes the glucose range was increased to 0–300 mmol/L (*), 0–600 mmol/L (**), or 0–1000 mmol/L with 25 mmol/L ATP (#).NO = data not obtainable due to the severity of the kinetic inactivation which prevented the data from reaching a plateau even with a 10-fold increase in glucose concentration.