Dissecting the Crosstalk between Bisulfite and Hypochlorous Acid in the Reaction-Based Fluorescence Detection with Dicyanovinyl-Based Probes

Fluorescent
probes bearing a reactive moiety of 1,1-dicyanovinyl
are known to detect several biological species including bisulfite
and hypochlorous acid, which, however, possess a selectivity issue
among those analytes. Structural modifications of the reactive group
for optimal steric and electron effects based on theoretical calculations
led us to address the selectivity issue, offering new reactive moieties
that provide complete analyte selectivity, including that between
bisulfite and hypochlorous acid, in cells as well as in solution.