Activated TLR7 or 21 recruits MyD88

Cloning and analysis of full length chicken MyD88 revealed an open reading frame which encodes a predicted protein sequence of 368 amino acids (aa). Two more chicken MyD88 isoforms with 299 and 376 residues are described in the literature [Qiu Y et al 2008, Lynn et al 2003]. The amino acid sequences of the chMyD88 368 and 376 residue isoforms are 99% identical, 97% identical to the 299 aa chMyD88 predicted peptide and 68% identical to human MyD88 (296 aa).<p>Chicken MyD88 is recruited to the activated hetero or homodimer of chTLR that is located either on plasma membrane (TLR4, TLR2/1, TLR5 and TLR15) or endosome membrane (TLR7 and TLR21).

通过对全长鸡MyD88的克隆与分析，揭示了其开放阅读框编码的预测蛋白质序列由368个氨基酸（aa）组成。文献[Qiu Y et al 2008, Lynn et al 2003]中描述了另外两种鸡MyD88异构体，分别含有299和376个残基。chMyD88 368和376残基异构体的氨基酸序列具有99%的同一性，97%与299个氨基酸的预测肽段chMyD88相同，并与人的MyD88（296个氨基酸）具有68%的同一性。鸡MyD88被招募至位于质膜（TLR4、TLR2/1、TLR5和TLR15）或内体膜（TLR7和TLR21）的激活异源或同源二聚体chTLR上。