Effects of inhibiting stearoyl-CoA desaturase (SCD) on the spatial transcriptome in a mouse model of Alzheimer's disease (AD)

Here, the effects of a stearoyl-CoA desaturase inhibitor (SCDi) on the mouse brain spatial transcriptome was investigated. More precisely, wild-type and 3xTg-AD female mice were infused in the brain left lateral ventricle with control (DMSO) or SCDi during 28 days (after the implantation of electrodes for electrocorticography and electromyography). Brains were sampled on the morning of Day 31 after the beginning of treatment, and immediately frozen. Brains were treated and libraries were prepared according to 10x Genomics protocols for Visium Spatial Gene Expression. Sequencing was conducted by Genome Quebec (Montreal, QC, Canada). Overall design: The mouse brain transcriptome was evaluated after a 28-day treatment with SDCi in female wild-type (WT) and 3xTg-AD mice that were kept under a 12-hour light:12-hour dark cycle. The same measurements were conducted in mice of the two genotypes receiving DMSO (controls/vehicule). The total number of samples for this project is thus 4, consisting of one WT female receiving SCDi, one WT female receiving the vehicle, one 3xTg-AD female receiving SCDi, and one 3xTg-AD female receiving the vehicle. The brain transcriptome was quantified using the Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics). The original fastq files are provided as raw data.