Real-time quantitative PCR analysis of human Vastus Lateralis muscle biopsies

Vastus Lateralis muscle biopsies were obtained before and after the training protocol from 6 healthy elderly male people. We chose custom TaqMan low density array cards (Life Technologies) preloaded with 48 selected genes related to the following pathways: myogenesis, apoptosis, protein anabolism/catabolism and antioxidant enzymes to quantitate their gene expression in human skeletal muscle. RT-PCR gene expression profiling. Vastus Lateralis muscle biopsies were obtained before and after the training protocol from 6 healthy elderly male people. From each biopsies, total RNA was purified and then cDNA was synthesized from it. Equal amount of cDNA from each donor was analysed for its gene expression. For each donor, post-training gene expression was compared to pre-training. In the end mean and standard error was calculated.