Pichia kudriavzevii strain:GS1-1 Genome sequencing. Pichia kudriavzevii strain:GS1-1

Whole-genome de novo sequencing of Pichia kudriavzevii GS1-1 for strain-specific genomic information.The transcriptome of Pichia kudriavzevii when glucose or citric acid was used as the sole carbon source was further analyzed, potential citrate transporters were mined, and the function of citrate transporter was verified, so that Pichia kudriavzevii was responsible for the transmembrane transport of citrate. At the same time, the 13C-citrate tracer metabolome technique was used to analyze the flow and flux of citrate metabolites in Pichia kudriavzevii , and the key nodes of citrate decomposition were experimentally verified, so as to construct a citrate metabolic network and analyze its Speed limit steps.