Summary of 12 RNA-Seq Libraries Constructed from Seed Coats of Clark and Harosoy Standard and Defective Seed Coat Isolines at Three Stages of Immature Seed Development.

The USDA line number and PI accession numbers by which the isolines are searchable in the USDA GRIN (Germplasm Resources Information Network) database are shown. The molecular nature is known for the I alleles [14], [15] that control distribution of pigment and the R[16], and T loci [17] that affect seed color phenotype. RNA was extracted from seed coats dissected from immature seed of the indicated fresh weight ranges. The total number of 75- or 100-nt raw reads from each RNA-Seq library is shown. The pictures of each phenotype of the defective seed coat lines are shown in Figure 1. The raw and processed data for the 12 sequencing samples is available as accession series GSE54903 at the National Center for Biotechnology Information. (Abbreviations: CS: Clark Standard, CD: Clark Defective, HS: Harosoy Standard, HD: Harosoy Defective).