Study on the effect and mechanism of c-Maf liquid-liquid separation in promoting MM cellular proliferation and drug resistance through Mtbp/c-Myc axis

Previous experiments have demonstrated that c-Maf can undergo liquid-liquid phase separation (LLPS) in MM. In order to search for the downstream target genes of c-Maf based on its LLPS regulation, we successfully constructed three kinds of cells with EV, c-Maf and already c-Maf-IDR mutation, and simultaneously subjected the three kinds of cells to ChIP sequencing (selecting the FLAG-tagged antibody), and then analysed them for the differential genes, thus screening the downstream targets of c-Maf based on its LLPS regulation. Overall design: Cells were human multiple myeloma cell line (ARP1). Group: EV, c-Maf, c-Maf-IDR mutation. Antibody: FLAG.