Transcriptome analysis on the dorsal hippocampus of Df1/+ mice

DiGeorge/22q11.2 deletion syndrome (22q11.2DS) confers the highest known genetic risk for schizophrenia, yet the molecular mechanisms linking chromosomal deletion to cognitive dysfunction and psychosis remain incompletely understood. The hippocampus, critical for contextual memory and consistently implicated in schizophrenia pathophysiology, represents a key neural substrate for investigating disease mechanisms. Here, we performed RNA sequencing of the dorsal hippocampus in Df1/+ mice, a genetic model of 22q11.2DS, together with behavioral testing of contextual fear memory. We applied a multilayer transcriptomic framework integrating over-representation analysis and gene set enrichment analysis to define molecular changes in biological process and evaluated translational convergence by comparing mouse results with postmortem hippocampal RNA-seq from individuals with schizophrenia.