RNA-seq Reveals Macrophage Polarization and Fibrosis Markers in AKI to CKD Transition After Ischemia-Reperfusion Injury

RNA-seq analysis of kidney samples from mice after ischemia-reperfusion injury (IRI) identified 1,109 genes with altered expression between mild (20 minutes) and severe (35 minutes) IRI groups. Among these, 974 genes were upregulated and 135 were downregulated in the severe group. The experimental design involved using C57Bl/6 mice to study the transition from acute kidney injury (AKI) to chronic kidney disease (CKD) following ischemia-reperfusion injury (IRI). Two groups were established based on the duration of ischemia: a mild IRI group with 20 minutes of ischemia and a severe IRI group with 35 minutes of ischemia. The mice were sacrificed 28 days post-IRI to assess the long-term effects of the injury.