Transposon sequencing of Xylella fastidiosa M23 in grapevine and almond

This study used transposon sequencing (Tnseq) to predict genes essential for Xylella fastidiosa growth in plants. Tn5 transposon mutant pools of Xylella fastidiosa M23 were inoculated into susceptible grapevines and almond plants, and re-isolated after ten weeks. These sequences are 3 replicates each of Tn5 mutant pools re-isolated from grapevine, almond, and from growth in rich culture medium (PD3).