Bulk RNA-seq of IMR90 fibroblasts in IR-induced senescence with siRNA knockdowns of CCND1, CDK4, CDK6 and controls

We profiled transcriptomes of IR-induced senescent IMR90 fibroblasts subjected to siRNA-mediated knockdown of CCND1, CDK4, CDK6 (and CDK4+6), alongside two non-targeting controls. Transfections occurred on days?4/5 and?7/8 post-IR; all samples were harvested day?10 for RNA extraction. Differential expression identifies kinase-specific roles in sustaining inflammatory and DNA damage responses in senescence. Overall design: Conditions (n=3 each): • Proliferating (No IR) • Senescent + NT1 • Senescent + NT2 • Senescent + CCND1 KD • Senescent + CDK4 KD • Senescent + CDK6 KD • Senescent + CDK4+CDK6 KD Harvest day?10 post-IR/transfections