PhoA-Asaia protein fusion plasmids expressed in Asaia.
收藏Figshare2016-02-23 更新2026-04-29 收录
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PhoA = alkaline phosphatase with no signal sequence. The other lanes have Asaia sequences fused to ‘phoA. Tsr = TonB-dependent siderophore receptor. Aap = amino acid permease. Tdr = TonB-dependent receptor plug. Gdh = glucose dehydrogenase. Ybp = YVTN beta-propeller repeat protein. Csy = cellulose synthase. Amu = aminomutase. Ptp = peptide transport permease. The top ELISA used an anti-PhoA-HRP antibody to detect the presence of the alkaline phosphatase protein. The bottom plate assay used a PNPP substrate, which turns yellow when cleaved by active PhoA.
创建时间：
2016-02-23
