Summary of the Nervous necrosis virus NNV detection results from the Linyuana grouper fish farm by integrated microfluidic chip analysis.

aLocations of the grouper fish farms are shown in Figure S2 and the fish samples were collected in 2008 and 2009.b+, NNV-positive samples by microfluidic chip analysis; −, NNV-negative samples by microfluidic chip analysis.cThe observation of viral nervous necrosis (VNN) clinical signs following sampling; +, groupers displayed VNN clinical signs; −, groupers did not display any clinical signs of disease; Clinical signs of VNN in larval-stage groupers were abnormal schooling and swimming behavior (whirling, spiraling) and loss of appetite.