Dense time-course gene expression profiling of the Drosophila melanogaster innate immune response

We perform a dense time-course RNA-seq analysis of the Drosophila innate immune response. Flies were injected with commercial lipopolysaccharide (LPS), a known non-pathogenic elicitor that can stimulate a robust yet transient immune response while avoiding the confounding effects from a growing and changing internal population of pathogens. Flies were sampled in duplicate for RNA-seq analysis pre-infection as a control and post-infection for 20 time points throughout 5 days (total of 42 samples).