Characterization of RNA cleavage and PFS recognition by CRISPR-Cas12a3 and Cas12a4 nucleases
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https://www.ncbi.nlm.nih.gov/sra/ERP171385
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资源简介：
To investigate protospacer-flanking sequence (PFS) recognition preferences of CRISPR-Cas12a3 and Cas12a4 nucleases, we screened a library of possible PFS sequences in Escherichia coli using plasmid interference assays. To determine RNA substrate preferences, we performed nanopore direct RNA sequencing of cell-free transcriptionâtranslation reactions and complementary in vitro assays.
创建时间：
2025-10-28
