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Effect of GTP or (p)ppGpp levels in toxin induced PI staining and dormancy.

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https://figshare.com/articles/dataset/_Effect_of_GTP_or_p_ppGpp_levels_in_toxin_induced_PI_staining_and_dormancy_/358053
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xylR-PXylA or xylR-PXylA ΔrelA or xylR-PXylAζY83C or xylR-PXylAζY83C ΔrelA were grown in MMS7. At ∼5×107 cells/ml−1 0.5% Xyla (to induce ζY83C expression) or 0.5 mg ml−1 Deca (to reduce GTP synthesis) or bothb, Xyl and Dec, were added and the culture was incubated for 120 min. fThe presence of ζY83C toxin is indicated by yes or no. gNumber of cells analyzed are shown in parentheses. hDue to poor growth of the ΔrelA strains CFUs were measured after two days of incubation. iThe CFUs were measured after 120 min of toxin induction by plating appropriate dilutions on LB plates. The results are the average of at least three independent experiments and are within a 10% standard error.
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2012-01-25
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