Gene expression of white and grey matter demyelinated areas and normal appearing matter from Multiple Sclerosis leukocortial lesions of human post-mortem tissue

Using laser capture microscopy, white (WM) and grey matter (GM) demyelinated areas and normal appearing matter was collected from histologically verified leukocortical lesions from snap-frozen human post mortem tissuederived from Multiple Sclerosis patients. Our data shows large differences in gene expression in WM and GM demyelinated areas (compared to their respective normal appearing matter) even when the demyelinated areas are spatially connected such as in leukocortical lesions. Thus, we show that WM demyelinated areas and GM demyelinated areas are distinct entities with distinct pathology. Therefore findings observed in WM demyelinated areas cannot be generalized to GM demyelinated areas. Overall design: Sequencing libraries of 11 leukocortical lesions (4 areas per lesion) were prepared with the Quant Seq 3' mRNA-Seq Library Prep Kit FWD Please note that, for clarification and ease of use, the records was updated with clinical information (in the 'Clinical_Info.xlsx' file, on April 25, 2022).