Source Data for Figure 6 of Jimenez et al. Methods 2020

Localization files for Figure 6 of:Jimenez, A., Friedl, K., Leterrier, C. (2020). About samples, giving examples: Optimized Single Molecule Localization Microscopy Methods 174(), 100-114. Multicolor STORM and DNA-PAINT images of immunostained COS cells. A. 3-color image obtained by sequential acquisition of actin by STORM (phalloidinAF647, gray in overlay), microtubules (two anti-α-tubulin antibodies, green on overlay) and clathrin-coated pits (anti-clathrin light chain, red on overlay) by DNAPAINT (Atto655 imager). Bottom panels are zoomed images corresponding to the highlighted area on the full image. B. 5-color image obtained in three steps: first DNA-PAINT of actin (phalloidin-Atto488, green in overlay), microtubules (two anti-α-tubulin antibodies, Atto565 imager, magenta on overlay) and mitochondria (anti TOM20, Atto655 imager, red on overlay), then DNA-PAINT of intermediate filaments (anti-vimentin, Cy3B imager, blue on overlay), then DNA-PAINT of clathrin (anti-clathrin light chain, Cy3B imager, cyan on overlay).

图6的本地化文件：Jimenez, A., Friedl, K., Leterrier, C. (2020). 论样本与实例：优化单分子定位显微镜方法。174(), 100-114。免疫染色COS细胞的彩色STORM和DNA-PAINT图像。图A展示了通过STORM（使用phalloidinAF647进行肌动蛋白的顺序采集，叠加图中为灰色）获得的3色图像，微管（使用两种anti-α-tubulin抗体，叠加图中为绿色）和笼状凹陷（使用anti-clathrin轻链，叠加图中为红色）通过DNAPAINT（Atto655成像仪）进行成像。底部面板为放大图像，对应于全图中被突出显示的区域。图B通过三个步骤获得了5色图像：首先使用DNA-PAINT对肌动蛋白（使用phalloidin-Atto488，叠加图中为绿色）进行成像，微管（使用两种anti-α-tubulin抗体，通过Atto565成像仪，叠加图中为洋红色）和线粒体（使用anti TOM20，通过Atto655成像仪，叠加图中为红色）进行成像，然后对中间丝（使用anti-vimentin，通过Cy3B成像仪，叠加图中为蓝色）进行DNA-PAINT成像，最后对笼状蛋白进行DNA-PAINT成像（使用anti-clathrin轻链，通过Cy3B成像仪，叠加图中为青色）。