Cytosolic copper concentration modulates spore germination and secondary metabolism in Streptomyces coelicolor

We studied the influence of copper in physiological and morphological differentiation of Streptomyces coelicolor. We demonstrate differences in phenotype (germination, growth rate, antibiotic production) and genetic expression between a strain mutated at copper chaperone CopZ (SCO2730::Tn5062), the wild-type strain and a wild-type strain sporulated in a media with 80µM CuSO4. These differences are correlated with the cytosolic copper. Our results demonstrate a pleiotropic effect of copper modulating S. coelicolor development. Overall design: Expression profiles from SCO2730::Tn5062 mutant, wild-type strain and a wild-type strain sporulated in a media with 80µM CuSO4 were obtained by Next Generation Sequencing from two biological replicates, in sucrose-free R5A culture media.