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Method to identify genomic targets of DNA binding proteins.

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PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC53882/
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We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds. IMAGES:
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National Academy of Sciences
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