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RNA degradation in bone marrow-derived dendritic cells after LPS stimulation

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https://www.ncbi.nlm.nih.gov/sra/DRP003214
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资源简介:
GM-CSF-induced bone marrow derived dendritic cells (BM-DCs) were stimulated with 100 microG / mL of LPS (Re595). At 0, 1/2, 1, 2, 3, 4, 6, and 8 hours after the stimulation, 10 microG / mL of actinomycin D (ActD) was added, and at 0, 1/2, 1, 2, 4 hours after ActD addition cells were subjected to RNA extraction. The total RNA was further subjected for RNA-sequencing.
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2017-09-17
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