S2 Datasheet -

(A) The values shown are the change in the fluorescence intensity of the regions indicated in Fig 2A for each brain analyzed. This value is calculated as the intensity of RFP fluorescence (neurons that show increased Ca2+ influx)/the intensity of GFP fluorescence (all neurons that express the CaMPARI construct). (B) The values shown are the change in the anti-Bruchpilot antibody staining intensity of the regions shown in Fig 2B minus the background fluorescence for each brain analyzed. (C–F) The first column is the age of the animals in days, while each subsequent column contains the survivorship values calculated for the fly population indicated at the top. The genotypes of the flies used were: (C) EB-GAL4 x w1118 (control) or EB-GAL4 x UAS- TRPA1 (EB neurons activated), (D) R2/R4d-GAL4 x w1118 (control) or R2/R4d-GAL4 x UAS-TRPA1 (R2/R4d neurons activated), (E) R4d-GAL4 x w1118 (control) or R4d-GAL4 x UAS-TRPA1 (R4d neurons activated), and (F) R2-GAL4 x w1118 (control) or R2-GAL4 x UAS-TRPA1 (R2 neurons activated). (XLSX)