Haptophyte_ref_alignment_284tax

Reference alignment comprising 281 haptophyte 18S rDNA sequences representing all cultured haptophyte species, environmental sequences forming novel clades, and the best BLAST hits in NCBI-nr to the Oslofjorden OTUs. Three outgroup sequences are included (AJ564771 Telonema subtilis, AY919672 Kathablepharis remigera, L28811 Chilomonas paramecium). The sequences from cultured species and environmental sequences that formed novel clades were aligned in MAFFT v.6 with the Q-INS-i strategy (Katoh and Toh 2008). BLAST hits in NCBI-nr that were not already present in the alignment were inserted into the existing alignment using the add-in function in MAFFT for full-length sequences, with method L-INS-1 (Katoh and Frith 2012).