James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12370, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Time series images showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

时序图像展示了蜻蜓科昆虫 Nephrotoma suturalis 初级精母细胞中动粒微管在减数第一次分裂期间的动力学变化。采用 Nikon Microphot SA 显微镜进行时间间隔偏振显微镜观察，该显微镜配备有液晶偏振光显微镜系统（LC-PolScope，CRi，马萨诸塞州沃本），配备 60x/1.4 PlanApo 油浸物镜，1.4 NA 油浸聚光镜，并配备 2.0x 放大镜头。使用 QImaging Retigo EXi CCD 相机每隔 15 秒捕捉一次图像。原始图像经过 5 帧算法（Shribak 和 Oldenbourg，2003）处理。本组集中包含了一部时序图像的电影。