Ke Hu (Indiana University, Bloomington) (2011) CIL:10521, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgMORN1 and mCherryFP-tagged Tgalpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Captured with CoolSnapHQ/ICX285. Deconvolved stack corresponds to raw data stack: CIL# 10520.
Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

一层人表皮成纤维细胞被感染了表达EGFP标记的TgMORN1和mCherryFP标记的Tgalpha1tubulin的弓形虫Toxoplasma gondii。图像使用Olympus IX70倒置显微镜上的API DeltaVision系统获取。实验目标镜头为Olympus 100X油浸物镜，数值孔径为1.35。滤光片：EGFP，激发波长450-490nm，发射波长500-540nm；mCherry，激发波长555-592nm，发射波长600-665nm。图像通过CoolSnapHQ/ICX285相机捕获。去卷积后的图像堆栈对应于原始数据堆栈：CIL# 10520。该图像为未发表图像，与PMID: 16518471和20140195中发表的图像相似。有关其他实验细节，请参阅相关出版物。