Evaluation of gene expression changes in tcs09 single and double mutants in the pneumococcal strain D39

In order to elucidate the regulatory role of pneumococcal TCS09, a transcriptomic approach was performed using RNA-seq from bacterial cultures in chemical defined medium of single and double tcs09 mutants. A total of 69 genes presenting significant expression changes were selected for further evaluation and discussion. These 69 genes were classified accordingly to their biochemical function. response regulator 09, histidine kinase 09 and a rr09-hk09 mutants were generated in a pneumococcal D39 background. Each individual mutant strain and its wild-type were cultivated in chemically defined medium until mid-log phase and collected for RNA isolation in quadruplicate