Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α. Homo sapiens

To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV). Overall design: Total RNA was extracted from SaOS- 2-ΔNp63α and SaOS-2-EV cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in ΔNp63α overexpressing SaOS- 2 cells and genes from control SaOS-2-EV cells.